For assembling stable nsp7Cnsp8 complex, purified nsp7 was incubated with nsp8 at a molar ratio of 1 1:1 for 5?h on ice. of being developed into one of the much-needed SARS-CoV-2 therapeutics. Ni-NTA (NTA) biosensors (Cat# 18-5101, ForteBio) at a concentration of 150?g/mL, resulting in a saturation response of 5C6?nm after 300?s. Subsequently, the loaded … Continue reading For assembling stable nsp7Cnsp8 complex, purified nsp7 was incubated with nsp8 at a molar ratio of 1 1:1 for 5?h on ice